The core activity of Spirochrome is the development and manufacture of fluorescent probes and reagents for live-cell fluorescence microscopy. These products enable researchers to examine living cells with minimal disturbance, thereby preserving the biological integrity of the cells. The company focuses on producing high-quality, photostable and cell-permeable fluorophores specifically designed for the demands of live-cell imaging.
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Live-cell fluorescence probes for DNA, actin, microtubules, Golgi, peroxisomes, lysosomes, mitochondria, plasma membrane and other target structures; fluorogenic, cell-permeable and for live-cell imaging.
HaloTag and SNAP-Tag ligands
Chloroalkane and benzylguanine ligands for HaloTag and SNAP-Tag labeling in cellular fluorescence experiments; available in multiple colours for live- and fixed-cell imaging.
Membrane voltage probes
Fluorescent reporters for plasma membrane and organelle membrane voltage, including Flipper-TR, Halo-Flipper, as well as ER-, Mito- and Lyso-Flipper variants.
Mitochondria and plasma membrane probes (PKmito & PKmem)
Fluorescence probes for live- and fixed-cell imaging of mitochondria and plasma membrane; low phototoxicity, high photostability and variants for different channels.
Actin imaging probes
Fluorescent actin probes for live-cell imaging and fast actin dynamics, including SPY-, SiR- and FastAct variants as well as the Actin_TestKit for multiple applications.
DNA and cell nucleus probes
Fluorescent DNA probes for imaging the cell nucleus and DNA in living cells, available in multiple colour ranges and as kit variants.
Microtubule probes
Fluorescent microtubule probes for live-cell imaging, complemented by coloured variants and kit products for different imaging conditions.
Expansion microscopy tools
Tools for expansion microscopy, in particular HAK-actin for reliable actin labeling with U-ExM, cryo-ExM and iU-ExM protocols.
Fluorophores and reagents for conjugation
SiR reagents and clickable derivatives such as carboxylic acid, NHS ester, maleimide, azide, alkyne, tetrazine and BCN for custom conjugations.
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